Vinculin antibody (AA 1001-1134)

Catalog No. ABIN719261

There are 7+ publications for this product available. The Rabbit Polyclonal anti-Vinculin antibody is suitable to detect Vinculin in samples from Human. It has been validated for WB, FACS, IHC (p), IHC (fro), IF (cc) and IF (p).

Quick Overview for Vinculin antibody (AA 1001-1134) (ABIN719261)

Target: Vinculin (VCL)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Vinculin antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-Vinculin Antibody

Binding Specificity: AA 1001-1134

Purpose: Vinculin Polyclonal Antibody

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Vinculin

Isotype: IgG

Application Details

Application Notes: WB(1:300-5000),

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Vinculin antibody (ABIN719261)

Yunes-Medina, Paciorkowski, Nuzbrokh, Johnson: "Depletion of transglutaminase 2 in neurons alters expression of extracellular matrix and signal transduction genes and compromises cell viability." in: Molecular and cellular neurosciences, Vol. 86, pp. 72-80, (2018) (PubMed).

Dhawan, Pan, Shie, Chu, Huang, Chen, Chen: "The Spatiotemporal Control of Osteoblast Cell Growth, Behavior, and Function Dictated by Nanostructured Stainless Steel Artificial Microenvironments." in: Nanoscale research letters, Vol. 12, Issue 1, pp. 86, (2017) (PubMed).

Jiang, Yang, Li, Wu, Meng, Li: "Aqueous extracts of Tribulus terrestris protects against oxidized low-density lipoprotein-induced endothelial dysfunction." in: Chinese journal of integrative medicine, Vol. 22, Issue 3, pp. 193-200, (2016) (PubMed).

Dhawan, Pan, Lee, Chu, Huang, Lin, Chen: "Spatial Control of Cell-Nanosurface Interactions by Tantalum Oxide Nanodots for Improved Implant Geometry." in: PLoS ONE, Vol. 11, Issue 6, pp. e0158425, (2016) (PubMed).

He, Meng, Yao, Jiang, Wu, Wu: "The essential role of inorganic substrate in the migration and osteoblastic differentiation of mesenchymal stem cells." in: Journal of the mechanical behavior of biomedical materials, Vol. 59, pp. 353-65, (2016) (PubMed).

Jiang, Sun, Li, Hu, Zhou, Jiang, Xu: "Calycosin-7-O-?-D-glucoside promotes oxidative stress-induced cytoskeleton reorganization through integrin-linked kinase signaling pathway in vascular endothelial cells." in: BMC complementary and alternative medicine, Vol. 15, pp. 315, (2015) (PubMed).

Li, Sun, Yang, Hu, Jiang: "Tanshinone II a protects against lipopolysaccharides-induced endothelial cell injury via Rho/Rho kinase pathway." in: Chinese journal of integrative medicine, Vol. 20, Issue 3, pp. 216-23, (2014) (PubMed).

Target Details for Vinculin

Target: Vinculin (VCL)

Alternative Name: Vinculin

Background:

Synonyms: MV, MVCL, CMD1W, CMH15, HEL114, Vinculin, Metavinculin, VCL

Background: Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.

Gene ID: 7414

UniProt: P18206

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2