ICMT antibody (C-Term)

Catalog No. ABIN7464497

This anti-ICMT antibody is a Rabbit Polyclonal antibody detecting ICMT in WB and IHC (p). Suitable for Human.

Quick Overview for ICMT antibody (C-Term) (ABIN7464497)

Target: ICMT (Isoprenylcysteine Carboxyl Methyltransferase (ICMT))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ICMT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ICMT Antibody

Binding Specificity: C-Term

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ICMT. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HeLa membrane extract (unboiled)

Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for ICMT

Target: ICMT (Isoprenylcysteine Carboxyl Methyltransferase (ICMT))

Alternative Name: isoprenylcysteine carboxyl methyltransferase

Background: Isoprenylcysteine carboxyl methyltransferase , HSTE14 , MST098 , MSTP098 , PCCMT , PCMT , PPMT,This gene encodes the third of three enzymes that posttranslationally modify isoprenylated C-terminal cysteine residues in certain proteins and target those proteins to the cell membrane. This enzyme localizes to the endoplasmic reticulum. Alternative splicing may result in other transcript variants, but the biological validity of those transcripts has not been determined. [provided by RefSeq]

Molecular Weight: 32 kDa

Gene ID: 23463

UniProt: O60725