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Vimentin antibody (pSer56)

The Mouse Monoclonal anti-Vimentin antibody has been validated for WB and IHC (p). It is suitable to detect Vimentin in samples from Human.
Catalog No. ABIN7464523

Quick Overview for Vimentin antibody (pSer56) (ABIN7464523)

Target

See all Vimentin (VIM) Antibodies
Vimentin (VIM)

Reactivity

  • 376
  • 130
  • 128
  • 63
  • 61
  • 49
  • 43
  • 38
  • 30
  • 22
  • 21
  • 13
  • 13
  • 8
  • 8
  • 6
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  • 3
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  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Human

Host

  • 199
  • 163
  • 11
  • 6
  • 6
  • 5
  • 2
  • 2
Mouse

Clonality

  • 202
  • 191
Monoclonal

Conjugate

  • 240
  • 19
  • 13
  • 12
  • 9
  • 7
  • 6
  • 5
  • 5
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  • 5
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
This Vimentin antibody is un-conjugated

Application

  • 327
  • 170
  • 126
  • 106
  • 94
  • 63
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  • 40
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  • 34
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  • 23
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  • 1
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Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone

GT11512
  • Binding Specificity

    • 34
    • 32
    • 17
    • 16
    • 12
    • 10
    • 8
    • 7
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
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    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
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    • 1
    • 1
    pSer56

    Cross-Reactivity

    Human

    Purification

    Affinity purified by Protein G.

    Immunogen

    Carrier-protein conjugated synthetic peptide surrounding phospho Ser56 of human Vimentin. The exact sequence is proprietary.

    Isotype

    IgG1
  • Application Notes

    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

    Comment

    Positive Control: HeLa(100 ng/ml Nocodazole treatment for 24 hr)

    Validation: Orthogonal

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    PBS, No Preservative

    Preservative

    Without preservative

    Storage

    4 °C,-20 °C

    Storage Comment

    Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • Target

    Vimentin (VIM)

    Alternative Name

    vimentin

    Background

    Vimentin,Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM]

    Molecular Weight

    54 kDa

    Gene ID

    7431

    UniProt

    P08670

    Pathways

    Caspase Cascade in Apoptosis
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