Vimentin antibody (pSer56)
Quick Overview for Vimentin antibody (pSer56) (ABIN7464523)
Target
See all Vimentin (VIM) AntibodiesReactivity
Host
Clonality
Conjugate
Application
Clone
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Binding Specificity
- pSer56
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Cross-Reactivity
- Human
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Purification
- Affinity purified by Protein G.
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Immunogen
- Carrier-protein conjugated synthetic peptide surrounding phospho Ser56 of human Vimentin. The exact sequence is proprietary.
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Isotype
- IgG1
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Application Notes
- WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
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Comment
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Positive Control: HeLa(100 ng/ml Nocodazole treatment for 24 hr)
Validation: Orthogonal
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- PBS, No Preservative
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Preservative
- Without preservative
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- Vimentin (VIM)
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Alternative Name
- vimentin
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Background
- Vimentin,Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM]
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Molecular Weight
- 54 kDa
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Gene ID
- 7431
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UniProt
- P08670
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Pathways
- Caspase Cascade in Apoptosis
Target
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