Glypican 3 antibody (C-Term)

Catalog No. ABIN7464572

This anti-Glypican 3 antibody is a Rabbit Polyclonal antibody detecting Glypican 3 in IF, WB, IHC (p) and ICC. Suitable for Human.

Quick Overview for Glypican 3 antibody (C-Term) (ABIN7464572)

Target: Glypican 3 (GPC3)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Glypican 3 antibody is un-conjugated

Application: Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-Glypican 3 Antibody

Binding Specificity: C-Term

Cross-Reactivity: Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human Glypican-3. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HepG2 (24 μg/ml Tunicamycin treatment for 16 hr)

Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.27 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for Glypican 3

Target: Glypican 3 (GPC3)

Alternative Name: glypican 3

Background: Glypican 3 , DGSX , GTR2-2 , MXR7 , OCI-5 , SDYS , SGB , SGBS , SGBS1,Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2009]

Molecular Weight: 66 kDa

Gene ID: 2719

UniProt: P51654

Pathways: Glycosaminoglycan Metabolic Process