E2F1 antibody (AA 223-332)

Catalog No. ABIN7464706

The Mouse Monoclonal anti-E2F1 antibody has been validated for WB and IP. It is suitable to detect E2F1 in samples from Human.

Quick Overview for E2F1 antibody (AA 223-332) (ABIN7464706)

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This E2F1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Clone: 6B5

Product Details anti-E2F1 Antibody

Binding Specificity: AA 223-332

Specificity: This antibody recognizes E2F-1 that was identified as a cellular protein with DNA binding activity associated with the adenovirus E2 gene promoter.

Cross-Reactivity: Human

Purification: Protein G purified

Immunogen: The region encoding the DP-1 binding site (amino acids 223-332) of E2F-1 expressed in E. coli.

Isotype: IgG1

Application Details

Application Notes: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: MCF-7 , E2F1-transfected 293T

Validation: Overexpression

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2.29 mg/mL

Buffer: PBS, No Preservative

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for E2F1

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Alternative Name: E2F transcription factor 1

Background: E2F transcription factor 1 , E2F-1 , RBAP1 , RBBP3 , RBP3,The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. [provided by RefSeq, Jul 2008]

Molecular Weight: 47 kDa

Gene ID: 1869

UniProt: Q01094

Pathways: p53 Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy