PAX6 antibody

Catalog No. ABIN7464769

The Rabbit Polyclonal anti-PAX6 antibody has been validated for WB, IF, IHC (p), ICC, IP and IHC (fro). It is suitable to detect PAX6 in samples from Human.

Quick Overview for PAX6 antibody (ABIN7464769)

Target: PAX6 (Paired Box 6 (PAX6))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAX6 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-PAX6 Antibody

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human PAX6. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. IP: 1:1000-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: mouse brain , rat brain , PAX6-transfected 293T

Validation: Orthogonal, Overexpression

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.92 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for PAX6

Target: PAX6 (Paired Box 6 (PAX6))

Alternative Name: paired box 6

Background: Paired box 6 , AN , AN2 , ASGD5 , D11S812E , FVH1 , MGDA , WAGR,This gene encodes paired box gene 6, one of many human homologs of the Drosophila melanogaster gene prd. In addition to the hallmark feature of this gene family, a conserved paired box domain, the encoded protein also contains a homeo box domain. Both domains are known to bind DNA, and function as regulators of gene transcription. This gene is expressed in the developing nervous system, and in developing eyes. Mutations in this gene are known to cause ocular disorders such as aniridia and Peter's anomaly. Alternatively spliced transcript variants encoding either the same or different isoform have been found for this gene. [provided by RefSeq]

Molecular Weight: 47 kDa

Gene ID: 5080

UniProt: P26367

Pathways: Peptide Hormone Metabolism, Carbohydrate Homeostasis