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NMT1 antibody (N-Term)

This Rabbit Polyclonal antibody specifically detects NMT1 in WB, IF, ICC and IHC (p). It exhibits reactivity toward Human.
Catalog No. ABIN7465185

Quick Overview for NMT1 antibody (N-Term) (ABIN7465185)

Target

See all NMT1 Antibodies
NMT1 (N-Myristoyltransferase 1 (NMT1))

Reactivity

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  • 2
  • 1
  • 1
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Human

Host

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Rabbit

Clonality

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Polyclonal

Conjugate

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This NMT1 antibody is un-conjugated

Application

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Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • Binding Specificity

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    N-Term

    Cross-Reactivity

    Human, Mouse, Rat

    Purification

    Purified by antigen-affinity chromatography.

    Immunogen

    Recombinant protein encompassing a sequence within the N-terminus region of human NMT1. The exact sequence is proprietary.

    Isotype

    IgG
  • Application Notes

    WB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

    Comment

    Positive Control: 293T , A431 , HeLa , HepG2

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.42 mg/mL

    Buffer

    1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

    Preservative

    ProClin

    Precaution of Use

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C,-20 °C

    Storage Comment

    Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • Target

    NMT1 (N-Myristoyltransferase 1 (NMT1))

    Alternative Name

    N-myristoyltransferase 1

    Background

    N-myristoyltransferase 1 , NMT,Myristate, a rare 14-carbon saturated fatty acid, is cotranslationally attached by an amide linkage to the N-terminal glycine residue of cellular and viral proteins with diverse functions. N-myristoyltransferase (NMT, EC 2.3.1.97) catalyzes the transfer of myristate from CoA to proteins. N-myristoylation appears to be irreversible and is required for full expression of the biologic activities of several N-myristoylated proteins, including the alpha subunit of the signal-transducing guanine nucleotide-binding protein (G protein) GO (GNAO1, MIM 139311) (Duronio et al., 1992 [PubMed 1570339]).[supplied by OMIM]

    Molecular Weight

    57 kDa

    Gene ID

    4836

    UniProt

    P30419

    Pathways

    Regulation of G-Protein Coupled Receptor Protein Signaling
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