NMT1 antibody (N-Term)
Quick Overview for NMT1 antibody (N-Term) (ABIN7465185)
Target
See all NMT1 AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- N-Term
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Cross-Reactivity
- Human, Mouse, Rat
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Purification
- Purified by antigen-affinity chromatography.
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Immunogen
- Recombinant protein encompassing a sequence within the N-terminus region of human NMT1. The exact sequence is proprietary.
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Isotype
- IgG
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Application Notes
- WB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
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Comment
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Positive Control: 293T , A431 , HeLa , HepG2
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.42 mg/mL
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Buffer
- 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300
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Preservative
- ProClin
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Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- NMT1 (N-Myristoyltransferase 1 (NMT1))
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Alternative Name
- N-myristoyltransferase 1
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Background
- N-myristoyltransferase 1 , NMT,Myristate, a rare 14-carbon saturated fatty acid, is cotranslationally attached by an amide linkage to the N-terminal glycine residue of cellular and viral proteins with diverse functions. N-myristoyltransferase (NMT, EC 2.3.1.97) catalyzes the transfer of myristate from CoA to proteins. N-myristoylation appears to be irreversible and is required for full expression of the biologic activities of several N-myristoylated proteins, including the alpha subunit of the signal-transducing guanine nucleotide-binding protein (G protein) GO (GNAO1, MIM 139311) (Duronio et al., 1992 [PubMed 1570339]).[supplied by OMIM]
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Molecular Weight
- 57 kDa
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Gene ID
- 4836
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UniProt
- P30419
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Pathways
- Regulation of G-Protein Coupled Receptor Protein Signaling
Target
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