RFC4 antibody

Catalog No. ABIN7465204

The Mouse Monoclonal anti-RFC4 antibody has been validated for WB, IF and ICC. It is suitable to detect RFC4 in samples from Human.

Quick Overview for RFC4 antibody (ABIN7465204)

Target: RFC4 (Replication Factor C (Activator 1) 4, 37kDa (RFC4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This RFC4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 1320

Product Details anti-RFC4 Antibody

Cross-Reactivity: Human, Mouse

Purification: Protein G purified

Immunogen: Recombinant protein encoding the full-length p37 subunit ofhuman RFC purified from E. coli.

Isotype: IgG1

Application Details

Application Notes: WB: 1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 293T , A431 , HeLa , HepG2

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, No Preservative

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for RFC4

Target: RFC4 (Replication Factor C (Activator 1) 4, 37kDa (RFC4))

Alternative Name: replication factor C subunit 4

Background: Replication factor C subunit 4 , A1 , RFC37,The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008]

Molecular Weight: 40 kDa

Gene ID: 5984

UniProt: P35249

Pathways: Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA