E2F1 antibody (AA 244-264)

Catalog No. ABIN7465668

This Mouse Monoclonal antibody specifically detects E2F1 in WB, IP, IF, IHC (p) and ICC. It exhibits reactivity toward Human.

Quick Overview for E2F1 antibody (AA 244-264) (ABIN7465668)

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This E2F1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Clone: 1-80E-11

Product Details anti-E2F1 Antibody

Binding Specificity: AA 244-264

Cross-Reactivity: Human, Mouse

Purification: Protein G purified

Immunogen: The region encoding the DNA binding domain (amino acids 244-264) of E2F-1 expressed in E. coli.

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: E2F-1 transfected Soas2 cells

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, No Preservative

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for E2F1

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Alternative Name: E2F transcription factor 1

Background: E2F transcription factor 1 , E2F-1 , RBAP1 , RBBP3 , RBP3,The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. [provided by RefSeq, Jul 2008]

Molecular Weight: 47 kDa

Gene ID: 1869

UniProt: Q01094

Pathways: p53 Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy