SF3A2 antibody

Catalog No. ABIN7465860

This Rabbit Polyclonal antibody specifically detects SF3A2 in WB, IF, IHC (p) and ICC. It exhibits reactivity toward Human.

Quick Overview for SF3A2 antibody (ABIN7465860)

Target: SF3A2 (Splicing Factor 3a, Subunit 2, 66kDa (SF3A2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SF3A2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-SF3A2 Antibody

Cross-Reactivity: Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human SAP62. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HeLa

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.33 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for SF3A2

Target: SF3A2 (Splicing Factor 3a, Subunit 2, 66kDa (SF3A2))

Alternative Name: splicing factor 3a subunit 2

Background: Splicing factor 3a subunit 2 , PRP11 , PRPF11 , SAP62 , SF3a66,This gene encodes subunit 2 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 2 interacts with subunit 1 through its amino-terminus while the single zinc finger domain of subunit 2 plays a role in its binding to the 15S U2 snRNP. Subunit 2 may also function independently of its RNA splicing function as a microtubule-binding protein. [provided by RefSeq]

Molecular Weight: 49 kDa

Gene ID: 8175

UniProt: Q15428

Pathways: Ribonucleoprotein Complex Subunit Organization