ERI1 antibody

Catalog No. ABIN7466500

The Rabbit Polyclonal anti-ERI1 antibody is suitable to detect ERI1 in samples from Human. It has been validated for WB.

Quick Overview for ERI1 antibody (ABIN7466500)

Target: ERI1 (Exoribonuclease 1 (ERI1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERI1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ERI1 Antibody

Cross-Reactivity: Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human ERI1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Jurkat , Raji , NCI-H929

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for ERI1

Target: ERI1 (Exoribonuclease 1 (ERI1))

Alternative Name: exoribonuclease 1

Background: Exoribonuclease 1 , 3'HEXO , HEXO , THEX1,RNA exonuclease that binds to the 3'-end of histone mRNAs and degrades them, suggesting that it plays an essential role in histone mRNA decay after replication. A 2' and 3'-hydroxyl groups at the last nucleotide of the histone 3'-end is required for efficient degradation of RNA substrates. Also able to degrade the 3'-overhangs of short interfering RNAs (siRNAs) in vitro, suggesting a possible role as regulator of RNA interference (RNAi). Requires for binding the 5'-ACCCA-3' sequence present in stem-loop structure. Able to bind other mRNAs. Required for 5.8S rRNA 3'-end processing. Also binds to 5.8s ribosomal RNA. Binds with high affinity to the stem-loop structure of replication-dependent histone pre-mRNAs.

Molecular Weight: 40 kDa

Gene ID: 90459

UniProt: Q8IV48