GAPDHS antibody

Catalog No. ABIN7466997

This Rabbit Polyclonal antibody specifically detects GAPDHS in WB, IHC (p), ICC and IF. It exhibits reactivity toward Human.

Quick Overview for GAPDHS antibody (ABIN7466997)

Target: GAPDHS (Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GAPDHS antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-GAPDHS Antibody

Cross-Reactivity: Human, Mouse, Zebrafish (Danio rerio)

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human GAPDHS. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: NIH-3T3 , MCF-7 , mouse testis

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.91 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for GAPDHS

Target: GAPDHS (Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS))

Alternative Name: glyceraldehyde-3-phosphate dehydrogenase, spermatogenic

Background: Glyceraldehyde-3-phosphate dehydrogenase, spermatogenic , GAPD2 , GAPDH-2 , GAPDS , HEL-S-278 , HSD-35,This gene encodes a protein belonging to the glyceraldehyde-3-phosphate dehydrogenase family of enzymes that play an important role in carbohydrate metabolism. Like its somatic cell counterpart, this sperm-specific enzyme functions in a nicotinamide adenine dinucleotide-dependent manner to remove hydrogen and add phosphate to glyceraldehyde 3-phosphate to form 1,3-diphosphoglycerate. During spermiogenesis, this enzyme may play an important role in regulating the switch between different energy-producing pathways, and it is required for sperm motility and male fertility. [provided by RefSeq]

Molecular Weight: 45 kDa

Gene ID: 26330

UniProt: O14556

Pathways: Regulation of Carbohydrate Metabolic Process