POLE antibody (N-Term)

Catalog No. ABIN7467192

This Rabbit Polyclonal antibody specifically detects POLE in IF, WB, IHC (p) and ICC. It exhibits reactivity toward Human.

Quick Overview for POLE antibody (N-Term) (ABIN7467192)

Target: POLE (Polymerase (DNA Directed), Epsilon, Catalytic Subunit (POLE))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLE antibody is un-conjugated

Application: Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-POLE Antibody

Binding Specificity: N-Term

Cross-Reactivity: Human, Mouse

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human POLE. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.34 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for POLE

Target: POLE (Polymerase (DNA Directed), Epsilon, Catalytic Subunit (POLE))

Alternative Name: DNA polymerase epsilon

Background: DNA polymerase epsilon, catalytic subunit , CRCS12 , FILS , IMAGEI , POLE1,Participates in DNA repair and in chromosomal DNA replication.

Molecular Weight: 262 kDa

Gene ID: 5426

UniProt: Q07864

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA