POLI antibody

Catalog No. ABIN7467214

The Rabbit Polyclonal anti-POLI antibody has been validated for WB, IF and ICC. It is suitable to detect POLI in samples from Human.

Quick Overview for POLI antibody (ABIN7467214)

Target: POLI (Polymerase (DNA Directed) iota (POLI))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLI antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-POLI Antibody

Cross-Reactivity: Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human DNA polymerase iota. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: PC-3

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for POLI

Target: POLI (Polymerase (DNA Directed) iota (POLI))

Alternative Name: DNA polymerase iota

Background: DNA polymerase iota , RAD30B , RAD3OB , eta2,Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.

Molecular Weight: 83 kDa

Gene ID: 11201

UniProt: Q9UNA4

Pathways: DNA Damage Repair