EIF3A antibody (N-Term)

Catalog No. ABIN7467315

This Rabbit Polyclonal antibody specifically detects EIF3A in WB, IF, IHC (p) and ICC. It exhibits reactivity toward Human.

Quick Overview for EIF3A antibody (N-Term) (ABIN7467315)

Target: EIF3A (Eukaryotic Translation Initiation Factor 3 Subunit A (EIF3A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF3A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-EIF3A Antibody

Binding Specificity: N-Term

Cross-Reactivity: Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human EIF3A. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Raji

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.96 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for EIF3A

Target: EIF3A (Eukaryotic Translation Initiation Factor 3 Subunit A (EIF3A))

Alternative Name: eukaryotic translation initiation factor 3 subunit A

Background: Eukaryotic translation initiation factor 3 subunit A , EIF3 , EIF3S10 , P167 , TIF32 , eIF3-p170 , eIF3-theta , p180 , p185,Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.

Molecular Weight: 167 kDa

Gene ID: 8661

UniProt: Q14152

Pathways: Ribonucleoprotein Complex Subunit Organization