WDR77 antibody

Catalog No. ABIN7467489

This Rabbit Polyclonal antibody specifically detects WDR77 in WB, IF, ICC and IHC (p). It exhibits reactivity toward Human.

Quick Overview for WDR77 antibody (ABIN7467489)

Target: WDR77 (WD Repeat Domain 77 (WDR77))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WDR77 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-WDR77 Antibody

Cross-Reactivity: Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human WDR77. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:5000-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: U87-MG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for WDR77

Target: WDR77 (WD Repeat Domain 77 (WDR77))

Alternative Name: WD repeat domain 77

Background: WD repeat domain 77 , HKMT1069 , MEP-50 , MEP50 , Nbla10071 , p44 , p44/Mep50,WDR77 is a component of the 20S PRMT5 (MIM 604045)-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins (see MIM 601061). This modification targets Sm proteins to the survival of motor neurons (SMN) complex (see MIM 600354) for assembly into small nuclear ribonucleoprotein core particles (Friesen et al., 2002 [PubMed 11756452]).[supplied by OMIM]

Molecular Weight: 37 kDa

Gene ID: 79084

UniProt: Q9BQA1

Pathways: Ribonucleoprotein Complex Subunit Organization