P4HA1 antibody (C-Term)

Catalog No. ABIN7467794

The Rabbit Polyclonal anti-P4HA1 antibody (ABIN7467794) specifically detects P4HA1 in WB and IHC (p). The antibody is reactive with Human samples.

Quick Overview for P4HA1 antibody (C-Term) (ABIN7467794)

Target: P4HA1 (Prolyl 4-Hydroxylase, alpha Polypeptide I (P4HA1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This P4HA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-P4HA1 Antibody

Binding Specificity: C-Term

Cross-Reactivity: Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human P4HA1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.18 mg/mL

Buffer: 1XPBS pH 7, 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for P4HA1

Target: P4HA1 (Prolyl 4-Hydroxylase, alpha Polypeptide I (P4HA1))

Alternative Name: prolyl 4-hydroxylase subunit alpha 1

Background: Prolyl 4-hydroxylase subunit alpha 1 , P4HA,This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq]

Molecular Weight: 61 kDa

Gene ID: 5033

UniProt: P13674