APEX1 antibody

Catalog No. ABIN7467961

The Mouse Monoclonal anti-APEX1 antibody is suitable to detect APEX1 in samples from Human. It has been validated for WB, ELISA and IHC (p).

Quick Overview for APEX1 antibody (ABIN7467961)

Target: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This APEX1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 2104

Product Details anti-APEX1 Antibody

Cross-Reactivity: Human, Mouse

Purification: Protein G purified

Immunogen: Full length human APE1 purified from E. coli

Isotype: IgG1

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:5000. ELISA: 1:1000-1:10000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HepG2, HeLa, Raji whole cell lysates

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.2, No Preservative

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for APEX1

Target: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Alternative Name: apurinic/apyrimidinic endodeoxyribonuclease 1

Background:

Synonyms: apurinic/apyrimidinic endodeoxyribonuclease 1 , APE , APE1 , APEN , APEX , APX , HAP1 , REF1

Background: Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene, all encode the same protein. [provided by RefSeq, Jul 2008]

Molecular Weight: 36 kDa

Gene ID: 328

UniProt: P27695

Pathways: DNA Damage Repair, Chromatin Binding, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Positive Regulation of Response to DNA Damage Stimulus