ARID1B antibody

Catalog No. ABIN7467972

This Rabbit Polyclonal antibody specifically detects ARID1B in WB. It exhibits reactivity toward Human.

Quick Overview for ARID1B antibody (ABIN7467972)

Target: ARID1B (AT Rich Interactive Domain 1B (SWI1-Like) (ARID1B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARID1B antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ARID1B Antibody

Cross-Reactivity: Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human ARID1B. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 293T , A431 , HeLa , HepG2

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.32 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for ARID1B

Target: ARID1B (AT Rich Interactive Domain 1B (SWI1-Like) (ARID1B))

Alternative Name: AT-rich interaction domain 1B

Background:

Synonyms: AT-rich interaction domain 1B , 6A3-5 , BAF250B , BRIGHT , CSS1 , DAN15 , ELD/OSA1 , MRD12 , OSA2 , P250R

Background: Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Binds DNA non-specifically.

Molecular Weight: 236 kDa

Gene ID: 57492

UniProt: Q8NFD5