ATG3 antibody

Catalog No. ABIN7468000

The Rabbit Polyclonal anti-ATG3 antibody has been validated for WB. It is suitable to detect ATG3 in samples from Human.

Quick Overview for ATG3 antibody (ABIN7468000)

Target: ATG3 (ATG3 Autophagy Related 3 (ATG3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG3 antibody is un-conjugated

Application: Western Blotting (WB)

Grade: KO Validated

Product Details anti-ATG3 Antibody

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human ATG3. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Jurkat , Raji , PC-12 , Rat-2 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , K562 , THP-1 , HL-60 , HeLa Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for ATG3

Target: ATG3 (ATG3 Autophagy Related 3 (ATG3))

Alternative Name: autophagy related 3

Background:

Synonyms: autophagy related 3 , APG3 , APG3-LIKE , APG3L , PC3-96

Background: Autophagy is a process of bulk degradation of cytoplasmic components by the lysosome or vacuole. Human ATG3 displays the same enzymatic characteristics in vitro as yeast Apg3, a protein-conjugating enzyme essential for autophagy (Tanida et al., 2002 [PubMed 11825910]).[supplied by OMIM]

Molecular Weight: 36 kDa

Gene ID: 64422

UniProt: Q9NT62

Pathways: Autophagy