Calnexin antibody (C-Term)

Catalog No. ABIN7468094

This anti-Calnexin antibody is a Rabbit Polyclonal antibody detecting Calnexin in WB, IF, IP, ICC and IHC (p). Suitable for Human.

Quick Overview for Calnexin antibody (C-Term) (ABIN7468094)

Target: Calnexin (CANX)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Calnexin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: KO Validated

Product Details anti-Calnexin Antibody

Binding Specificity: C-Term

Specificity: IP/MS validation was supported by references (PMID:30377396)

Cross-Reactivity: Human, Mouse, Rat, Sheep

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Calnexin. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:5000-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:500-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Neuro2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , PC-12 , Rat2 , 293T Validation: Comparison, IP/MS, KO/KD, Orthogonal, Overexpression

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.46 mg/mL

Buffer: 1XPBS pH 7, 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for Calnexin

Target: Calnexin (CANX)

Alternative Name: calnexin

Background:

Synonyms: calnexin , CNX , IP90 , P90

Background: This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq]

Molecular Weight: 68 kDa

Gene ID: 821

UniProt: P27824

Pathways: MAPK Signaling, Thyroid Hormone Synthesis