EGLN1 antibody (C-Term)

Catalog No. ABIN7468296

This Rabbit Polyclonal antibody specifically detects EGLN1 in WB, IF, ICC and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for EGLN1 antibody (C-Term) (ABIN7468296)

Target: EGLN1 (Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EGLN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-EGLN1 Antibody

Binding Specificity: C-Term

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PHD2. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-Fr: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HeLa(200 μM CoCl2 treatment for 24 hr) Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.99 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for EGLN1

Target: EGLN1 (Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1))

Alternative Name: egl-9 family hypoxia inducible factor 1

Background:

Synonyms: egl-9 family hypoxia inducible factor 1 , C1orf12 , ECYT3 , HALAH , HIF-PH2 , HIFPH2 , HPH-2 , HPH2 , PHD2 , SM20 , ZMYND6

Background: The protein encoded by this gene catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. HIF is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. This protein functions as a cellular oxygen sensor, and under normal oxygen concentration, modification by prolyl hydroxylation is a key regulatory event that targets HIF subunits for proteasomal destruction via the von Hippel-Lindau ubiquitylation complex. Mutations in this gene are associated with erythrocytosis familial type 3 (ECYT3). [provided by RefSeq]

Molecular Weight: 46 kDa

Gene ID: 54583

UniProt: Q9GZT9

Pathways: cAMP Metabolic Process, Warburg Effect