EWSR1 antibody

Catalog No. ABIN7468326

This Rabbit Polyclonal antibody specifically detects EWSR1 in WB, IF, ICC, IHC (p) and IP. It exhibits reactivity toward Human.

Quick Overview for EWSR1 antibody (ABIN7468326)

Target: EWSR1 (Ewing Sarcoma Breakpoint Region 1 (EWSR1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EWSR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Product Details anti-EWSR1 Antibody

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human EWSR1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 293T , HeLa , HepG2

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.66 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for EWSR1

Target: EWSR1 (Ewing Sarcoma Breakpoint Region 1 (EWSR1))

Alternative Name: EWS RNA binding protein 1

Background:

Synonyms: EWS RNA binding protein 1 , EWS , EWS-FLI1 , bK984G1.4

Background: This gene encodes a putative RNA binding protein. Mutations in this gene, specifically a t(11,22)(q24,q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors. Alternative splicing of this gene results in two products. [provided by RefSeq]

Molecular Weight: 68 kDa

Gene ID: 2130

UniProt: Q01844