IDH1 antibody

Catalog No. ABIN7468500

This Mouse Monoclonal antibody specifically detects IDH1 in WB, IP, IF and ICC. It exhibits reactivity toward Human.

Quick Overview for IDH1 antibody (ABIN7468500)

Target: IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IDH1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)

Grade: KO Validated

Clone: GT1521

Product Details anti-IDH1 Antibody

Cross-Reactivity: Human, Mouse, Rat

Purification: Affinity purified by Protein G.

Immunogen: Recombinant protein encompassing a sequence within the center region of human IDH1. The exact sequence is proprietary.

Isotype: IgG2b

Application Details

Application Notes: WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IP: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 293T , A431 , HeLa , HepG2 , PC-12 , Rat-2 , Neuro2A , GL261 , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , Huh-7 Validation: Comparison, KO/KD, Overexpression

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, No Preservative

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for IDH1

Target: IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))

Alternative Name: isocitrate dehydrogenase (NADP(+)) 1, cytosolic

Background:

Synonyms: isocitrate dehydrogenase (NADP(+)) 1, cytosolic , HEL-216 , HEL-S-26 , IDCD , IDH , IDP , IDPC , PICD

Background: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. [provided by RefSeq]

Molecular Weight: 47 kDa

Gene ID: 3417

UniProt: O75874

Pathways: Warburg Effect