NEK1 antibody

Catalog No. ABIN7468661

This anti-NEK1 antibody is a Rabbit Polyclonal antibody detecting NEK1 in WB, IF, IP and ICC. Suitable for Human.

Quick Overview for NEK1 antibody (ABIN7468661)

Target: NEK1 (NIMA-Related Kinase 1 (NEK1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Grade: KO Validated

Product Details anti-NEK1 Antibody

Cross-Reactivity: Human, Mouse

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Nek1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 293T , mouse brain , rat brain Validation: KO/KD, Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.33 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for NEK1

Target: NEK1 (NIMA-Related Kinase 1 (NEK1))

Alternative Name: NIMA related kinase 1

Background:

Synonyms: NIMA related kinase 1 , ALS24 , NY-REN-55 , SRPS2 , SRPS2A , SRTD6

Background: The protein encoded by this gene is a serine/threonine kinase involved in cell cycle regulation. The encoded protein is found in a centrosomal complex with FEZ1, a neuronal protein that plays a role in axonal development. Defects in this gene are a cause of polycystic kidney disease (PKD). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2010]

Molecular Weight: 143 kDa

Gene ID: 4750

UniProt: Q96PY6