POLR2E antibody

Catalog No. ABIN7468766

The Rabbit Polyclonal anti-POLR2E antibody (ABIN7468766) specifically detects POLR2E in WB, IF and ICC. The antibody is reactive with Human samples.

Quick Overview for POLR2E antibody (ABIN7468766)

Target: POLR2E (Polymerase (RNA) II (DNA Directed) Polypeptide E, 25kDa (POLR2E))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR2E antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-POLR2E Antibody

Cross-Reactivity: Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human POLR2E. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: A549

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.49 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for POLR2E

Target: POLR2E (Polymerase (RNA) II (DNA Directed) Polypeptide E, 25kDa (POLR2E))

Alternative Name: RNA polymerase II subunit E

Background:

Synonyms: RNA polymerase II subunit E , RPABC1 , RPB5 , XAP4 , hRPB25 , hsRPB5

Background: This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11. [provided by RefSeq]

Molecular Weight: 25 kDa

Gene ID: 5434

UniProt: P19388

Pathways: Regulatory RNA Pathways