POLR2E antibody

Catalog No. ABIN7468766

Product Details anti-POLR2E Antibody

Target: POLR2E (Polymerase (RNA) II (DNA Directed) Polypeptide E, 25kDa (POLR2E))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR2E antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Cross-Reactivity: Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human POLR2E. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: A549

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.49 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for POLR2E

Target: POLR2E (Polymerase (RNA) II (DNA Directed) Polypeptide E, 25kDa (POLR2E))

Alternative Name: RNA polymerase II subunit E (POLR2E Products)

Synonyms: MGC68604 antibody, polr2e antibody, wu:fk97g10 antibody, zgc:101098 antibody, 2410021N14Rik antibody, 25kDa antibody, AW208866 antibody, RPB5 antibody, XAP4 antibody, RPABC1 antibody, hRPB25 antibody, hsRPB5 antibody, polymerase (RNA) II (DNA directed) polypeptide E, 25kDa S homeolog antibody, RNA polymerase II subunit E antibody, polymerase (RNA) II (DNA directed) polypeptide E, b antibody, polymerase (RNA) II (DNA directed) polypeptide E, 25kDa antibody, polymerase (RNA) II (DNA directed) polypeptide E antibody, DNA-directed RNA polymerases I, II, and III subunit RPABC1 antibody, polr2e.S antibody, POLR2E antibody, polr2eb antibody, polr2e antibody, Polr2e antibody, rpb-5 antibody

Background:

Synonyms: RNA polymerase II subunit E , RPABC1 , RPB5 , XAP4 , hRPB25 , hsRPB5

Background: This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11. [provided by RefSeq]

Molecular Weight: 25 kDa

Gene ID: 5434

UniProt: P19388

Pathways: Regulatory RNA Pathways