RAD52 antibody

Catalog No. ABIN7468852

The Mouse Monoclonal anti-RAD52 antibody has been validated for WB. It is suitable to detect RAD52 in samples from Human.

Quick Overview for RAD52 antibody (ABIN7468852)

Target: RAD52 (RAD52 Homolog (S. Cerevisiae) (RAD52))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This RAD52 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 5H9

Product Details anti-RAD52 Antibody

Cross-Reactivity: Human

Purification: Protein G purified

Immunogen: Human Rad52 recombinant protein expressed in E. coli.

Isotype: IgG1

Application Details

Application Notes: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HeLa whole cell lysate

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS pH 7, 20 % Glycerol, No Preservative

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for RAD52

Target: RAD52 (RAD52 Homolog (S. Cerevisiae) (RAD52))

Alternative Name: RAD52 homolog, DNA repair protein

Background:

Synonyms: RAD52 homolog, DNA repair protein

Background: The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. A pseudogene of this gene is present on chromosome 2. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Jul 2014]

Molecular Weight: 46 kDa

Gene ID: 5893

UniProt: P43351

Pathways: DNA Damage Repair