SIN3A antibody (N-Term)

Catalog No. ABIN7468972

The Rabbit Polyclonal anti-SIN3A antibody has been validated for WB, IF, IP, ChIP, ICC and IHC (p). It is suitable to detect SIN3A in samples from Human.

Quick Overview for SIN3A antibody (N-Term) (ABIN7468972)

Target: SIN3A (SIN3 homolog A, transcription regulator (SIN3A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIN3A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: KO Validated

Product Details anti-SIN3A Antibody

Binding Specificity: N-Term

Specificity: Knockdown/Knockout validation was supported by references (PMID:28805661)

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human SIN3A. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 293T , A431 , HeLa , HepG2 , mouse brain , rat brain Validation: KO/KD, Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.38 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for SIN3A

Target: SIN3A (SIN3 homolog A, transcription regulator (SIN3A))

Alternative Name: SIN3 transcription regulator family member A

Background:

Synonyms: SIN3 transcription regulator family member A , WITKOS

Background: The protein encoded by this gene is a transcriptional regulatory protein. It contains paired amphipathic helix (PAH) domains, which are important for protein-protein interactions and may mediate repression by the Mad-Max complex. [provided by RefSeq]

Molecular Weight: 145 kDa

Gene ID: 25942

UniProt: Q96ST3

Pathways: Activation of Innate immune Response, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha