VAV1 antibody (pTyr174)

Catalog No. ABIN7469173

The Rabbit Polyclonal anti-VAV1 antibody is suitable to detect VAV1 in samples from Human. It has been validated for WB.

Quick Overview for VAV1 antibody (pTyr174) (ABIN7469173)

Target: VAV1 (Vav 1 Oncogene (VAV1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VAV1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-VAV1 Antibody

Binding Specificity: pTyr174

Cross-Reactivity: Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide surrounding phospho Tyr174 of human VAV1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Jurkat (serum starvation for 15 min then 0.13 μg/mL anti-T-cell receptor antibody (clone 305) treatment for 5 min) Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.23 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for VAV1

Target: VAV1 (Vav 1 Oncogene (VAV1))

Alternative Name: vav guanine nucleotide exchange factor 1

Background:

Synonyms: vav guanine nucleotide exchange factor 1 , VAV

Background: The protein encoded by this proto-oncogene is a member of the Dbl family of guanine nucleotide exchange factors (GEF) for the Rho family of GTP binding proteins. The protein is important in hematopoiesis, playing a role in T-cell and B-cell development and activation. This particular GEF has been identified as the specific binding partner of Nef proteins from HIV-1. Coexpression and binding of these partners initiates profound morphological changes, cytoskeletal rearrangements and the JNK/SAPK signaling cascade, leading to increased levels of viral transcription and replication. [provided by RefSeq]

Molecular Weight: 98 kDa

Gene ID: 7409

UniProt: P15498

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, CXCR4-mediated Signaling Events, BCR Signaling