BCAR1 antibody (pTyr410)

Catalog No. ABIN7469254

The Rabbit Polyclonal anti-BCAR1 antibody is suitable to detect BCAR1 in samples from Human. It has been validated for WB, IF and ICC.

Quick Overview for BCAR1 antibody (pTyr410) (ABIN7469254)

Target: BCAR1 (Breast Cancer Anti-Estrogen Resistance 1 (BCAR1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BCAR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-BCAR1 Antibody

Binding Specificity: pTyr410

Cross-Reactivity: Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide surrounding phospho Tyr410 of human p130Cas. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HeLa Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.58 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for BCAR1

Target: BCAR1 (Breast Cancer Anti-Estrogen Resistance 1 (BCAR1))

Alternative Name: BCAR1

Background:

Synonyms: BCAR1 scaffold protein, Cas family member , CAS , CAS1 , CASS1 , CRKAS , P130Cas

Background: BCAR1, or CAS, is an Src (MIM 190090) family kinase substrate involved in various cellular events, including migration, survival, transformation, and invasion (Sawada et al., 2006 [PubMed 17129785]).[supplied by OMIM]

Molecular Weight: 93 kDa

Gene ID: 9564

UniProt: P56945

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, CXCR4-mediated Signaling Events, Platelet-derived growth Factor Receptor Signaling