MBD4 antibody

Catalog No. ABIN7469292

This Rabbit Polyclonal antibody specifically detects MBD4 in WB, IF and ICC. It exhibits reactivity toward Human.

Quick Overview for MBD4 antibody (ABIN7469292)

Target: MBD4 (Methyl-CpG Binding Domain Protein 4 (MBD4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MBD4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-MBD4 Antibody

Cross-Reactivity: Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human MBD4. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 3xFlag-human MBD4-transfected 293T cells

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for MBD4

Target: MBD4 (Methyl-CpG Binding Domain Protein 4 (MBD4))

Alternative Name: MBD4

Background:

Synonyms: methyl-CpG binding domain 4, DNA glycosylase , MED1

Background: DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MBD4 may function to mediate the biological consequences of the methylation signal. In addition, MBD4 has protein sequence similarity to bacterial DNA repair enzymes and thus may have some function in DNA repair. Further, MBD4 gene mutations are detected in tumors with primary microsatellite-instability (MSI), a form of genomic instability associated with defective DNA mismatch repair, and MBD4 gene meets 4 of 5 criteria of a bona fide MIS target gene. [provided by RefSeq]

Molecular Weight: 66 kDa

Gene ID: 8930

UniProt: O95243

Pathways: DNA Damage Repair