Histone H2B antibody (acLys20)

Catalog No. ABIN7469398

The Rabbit Polyclonal anti-Histone H2B antibody is suitable to detect Histone H2B in samples from Human. It has been validated for WB, IF, ICC, IP and DB.

Quick Overview for Histone H2B antibody (acLys20) (ABIN7469398)

Target: Histone H2B

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H2B antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP), Dot Blot (DB)

Product Details anti-Histone H2B Antibody

Binding Specificity: acLys20

Cross-Reactivity: Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide surrounding acetyl Lys20 of human Histone H2B. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. IP: 1:100-1:5000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HeLa (0.012% DMSO treatment for 18 hr) , HeLa (0.4 μM Trichostatin A treatment for 18 hr) Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for Histone H2B

Target: Histone H2B