NUF2 antibody

Catalog No. ABIN7469514

This Mouse Monoclonal antibody specifically detects NUF2 in WB, IP and IHC (p). It exhibits reactivity toward Human.

Quick Overview for NUF2 antibody (ABIN7469514)

Target: NUF2 (NUF2, NDC80 Kinetochore Complex Component (NUF2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NUF2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: GT312

Product Details anti-NUF2 Antibody

Cross-Reactivity: Human

Purification: Affinity purified by Protein G.

Immunogen: Recombinant protein corresponding to human NUF2

Isotype: IgG1

Application Details

Application Notes: WB: 1:500-1:3000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HeLa

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, No Preservative

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for NUF2

Target: NUF2 (NUF2, NDC80 Kinetochore Complex Component (NUF2))

Alternative Name: NUF2

Background:

Synonyms: NUF2 component of NDC80 kinetochore complex , CDCA1 , CT106 , NUF2R

Background: This gene encodes a protein that is highly similar to yeast Nuf2, a component of a conserved protein complex associated with the centromere. Yeast Nuf2 disappears from the centromere during meiotic prophase when centromeres lose their connection to the spindle pole body, and plays a regulatory role in chromosome segregation. The encoded protein is found to be associated with centromeres of mitotic HeLa cells, which suggests that this protein is a functional homolog of yeast Nuf2. Alternatively spliced transcript variants that encode the same protein have been described. [provided by RefSeq]

Molecular Weight: 54 kDa

Gene ID: 83540

UniProt: Q9BZD4

Pathways: Maintenance of Protein Location