TLE1 antibody

Catalog No. ABIN7469631

This Rabbit Polyclonal antibody specifically detects TLE1 in WB, IF and ICC. It exhibits reactivity toward Human.

Quick Overview for TLE1 antibody (ABIN7469631)

Target: TLE1 (Transducin-Like Enhancer of Split 1 (E(sp1) Homolog, Drosophila) (TLE1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TLE1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Grade: KO Validated

Product Details anti-TLE1 Antibody

Cross-Reactivity: Human, Rat

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human TLE1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 293T Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.92 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for TLE1

Target: TLE1 (Transducin-Like Enhancer of Split 1 (E(sp1) Homolog, Drosophila) (TLE1))

Alternative Name: TLE1

Background:

Synonyms: TLE family member 1, transcriptional corepressor , ESG , ESG1 , GRG1

Background: Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG.

Molecular Weight: 83 kDa

Gene ID: 7088

UniProt: Q04724

Pathways: WNT Signaling, SARS-CoV-2 Protein Interactome