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Recombinant CD4 antibody (AA 216-396)

The Rabbit Monoclonal anti-CD4 antibody (Clone 004R) (ABIN7470577) specifically detects CD4 in IHC. The antibody is reactive with Human samples.

$In tonsil tissue a strong CD4 immunostaining is seen in a fraction of T cells and in Langerhans cells of the tonsil surface epithelium. (Recombinant CD4 antibody (AA 216-396))$ IHC

B CLL containing numerous CD4 positive lymphocytes (Recombinant CD4 antibody (AA 216-396))

IHC

liver (Recombinant CD4 antibody (AA 216-396))

IHC

3 images

Catalog No. ABIN7470577

$503.00

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Quick Overview for Recombinant CD4 antibody (AA 216-396) (ABIN7470577)

Target

See all CD4 Antibodies

CD4

Antibody Type

Recombinant Antibody

Reactivity

Human

Host

Rabbit

Clonality

Monoclonal

Conjugate

This CD4 antibody is un-conjugated

Application

Immunohistochemistry (IHC)

Clone

004R

Discover our top product CD4 Primary Antibody

Binding Specificity
27

16

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16

12

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11

9

9

8

4

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3

3

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2

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2

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1
AA 216-396

Purpose

CD4 antibody (004R)

Immunogen

Recombinant fragment (around aa 216-396) of human CD4 protein (exact sequence is proprietary)

Isotype

IgG
anti-CD4 (CD4) (AA 385-457) antibody
CD4 Reactivity: Human, Mouse, Rat, Chicken, Pig FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

anti-CD4 (CD4) antibody
CD4 Reactivity: Human, Mouse, Rat, Chicken, Monkey, Dog, Pig, Rabbit, Cow, Sheep, Guinea Pig, Goat, Horse, Avian, Donkey, Hamster WB, IHC (fro), IHC (p) Host: Goat Polyclonal unconjugated

anti-CD4 (CD4) (AA 231-330) antibody
CD4 Reactivity: Human, Mouse, Rat FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

anti-CD4 (CD4) (N-Term) antibody
CD4 Reactivity: Human FACS, WB, MS Host: Mouse Monoclonal MEM-241 unconjugated

anti-CD4 (CD4) antibody
CD4 Reactivity: Human, Mouse, Rat IF, IHC (p) Host: Mouse Monoclonal unconjugated

anti-CD4 (CD4) antibody
CD4 Reactivity: Mouse FACS, IP, IHC (fro), ICC, Func Host: Rat Monoclonal GK1-5 unconjugated

anti-CD4 (CD4) (AA 26-200) antibody
CD4 Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated

anti-CD4 (CD4) (AA 301-400) antibody
CD4 Reactivity: Human, Mouse, Rat FACS, WB, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-CD4 (CD4) (N-Term) antibody (FITC)
CD4 Reactivity: Human FACS Host: Mouse Monoclonal MEM-241 FITC

anti-CD4 (CD4) (N-Term) antibody (PE)
CD4 Reactivity: Human FACS Host: Mouse Monoclonal MEM-241 PE

Application Notes

IHC 1:100-1:200

Comment

Positive Control: Tonsil: A moderate to strong membranous CD4 immunostaining should be seen in a subset of T-lymphocytes which is predominantly located in the interfollicular area. Macrophages in the germinal centres should show a moderate CD4 positivity. Liver: Kupffer cells and endothelial cells in the liver sinusoids must show at least a moderate CD4 staining.
Product Description: CD4 antibody validated for immunohistochemistry on 76 different Normal Tissues
Negative Control: Tonsil: B-cells and epithelial cells must not show any CD4 staining. Liver: Hepatocytes must not show any CD4 staining.

Protocol

Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes.

Restrictions

For Research Use only
Format

Liquid

Storage

4 °C,-20 °C,-80 °C

Storage Comment

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 ° C. Antibody is stable for 24 months. Non- hazardous.

Expiry Date

24 months
Target

CD4

Alternative Name

CD4

Background

Gene Alias: L3T4, Leu3, Ly-4, Lymphocyte antigen CD4, p55, T cell antigen T4/LEU3, T cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

UniProt

P01730

Pathways

TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events