Recombinant CD4 antibody (AA 216-396)
Quick Overview for Recombinant CD4 antibody (AA 216-396) (ABIN7477393)
Target
See all CD4 AntibodiesAntibody Type
Reactivity
Host
Clonality
Conjugate
Application
Clone
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Binding Specificity
- AA 216-396
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Immunogen
- Recombinant fragment (around aa 216-396) of human CD4 protein (exact sequence is proprietary)
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Isotype
- IgG
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anti-CD4 (CD4) (AA 385-457) antibody
CD4 Reactivity: Human, Mouse, Rat, Chicken, Pig FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated
anti-CD4 (CD4) antibodyCD4 Reactivity: Human, Mouse, Rat, Chicken, Monkey, Dog, Pig, Rabbit, Cat, Cow, Sheep, Guinea Pig, Goat, Horse, Avian, Donkey, Hamster WB, IHC Host: Goat Polyclonal unconjugated
anti-CD4 (CD4) (AA 231-330) antibodyCD4 Reactivity: Human, Mouse, Rat FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated
anti-CD4 (CD4) (N-Term) antibodyCD4 Reactivity: Human FACS, WB, MS Host: Mouse Monoclonal MEM-241 unconjugated
anti-CD4 (CD4) antibodyCD4 Reactivity: Mouse FACS, IP, IHC (fro), ICC, Func Host: Rat Monoclonal GK1-5 unconjugated
anti-CD4 (CD4) (AA 26-200) antibodyCD4 Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated
anti-CD4 (CD4) (AA 301-400) antibodyCD4 Reactivity: Human, Mouse, Rat FACS, WB, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-CD4 (CD4) (N-Term) antibody (FITC)CD4 Reactivity: Human FACS Host: Mouse Monoclonal MEM-241 FITC
anti-CD4 (CD4) antibody (PE)CD4 Reactivity: Rat FACS Host: Mouse Monoclonal OX-35 PE
anti-CD4 (CD4) (N-Term) antibody (PE)CD4 Reactivity: Human FACS Host: Mouse Monoclonal MEM-241 PE
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Application Notes
- IHC 1:100-1:200
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Comment
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Positive Control: Tonsil: A moderate to strong membranous CD4 immunostaining should be seen in a subset of T-lymphocytes which is predominantly located in the interfollicular area. Macrophages in the germinal centres should show a moderate CD4 positivity. Liver: Kupffer cells and endothelial cells in the liver sinusoids must show at least a moderate CD4 staining.
Negative Control: Tonsil: B-cells and epithelial cells must not show any CD4 staining. Liver: Hepatocytes must not show any CD4 staining.
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Protocol
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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Restrictions
- For Research Use only
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Format
- Liquid
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- CD4
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Alternative Name
- CD4
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Background
- L3T4, Leu3, Ly-4, Lymphocyte antigen CD4, p55, T cell antigen T4/LEU3, T cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4,CD4 antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- P01730
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Pathways
- TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events
Target
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