Recombinant Cytokeratin 7 antibody (AA 1-100)

Catalog No. ABIN7477437

This Rabbit Monoclonal antibody specifically detects Cytokeratin 7 in IHC. It exhibits reactivity toward Human.

Quick Overview for Recombinant Cytokeratin 7 antibody (AA 1-100) (ABIN7477437)

Target: Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Cytokeratin 7 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-607R

Product Details anti-Cytokeratin 7 Antibody

Binding Specificity: AA 1-100

Immunogen: Recombinant fragment (around aa1-100) of human KRT7 protein (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Pancreas: CK7 staining is at least weak to moderate cytoplasmic intercalating ducts and strong in large pancreatic ducts.

Negative Control: Pancreas: CK7 staining should be absent in all acinar cells.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for Cytokeratin 7

Target: Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))

Alternative Name: Cytokeratin 7

Background: CK-7, K2C7, Keratin 55K Type II Cytoskeletal, Keratin Simple Epithelial Type 1 K7, Keratin Type II Cytoskeletal 7, Krt2-7, KRT7, Sarcolectin, SCL, Type II Mesothelial Keratin K7, Type-II Keratin Kb7,Cytokeratin 7 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P08729