Recombinant KRT14 antibody (AA 350-472)

Catalog No. ABIN7477443

Product Details anti-KRT14 Antibody

Target: KRT14 (Keratin 14 (KRT14))

Antibody Type: Recombinant Antibody

Binding Specificity: AA 350-472

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This KRT14 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Immunogen: Recombinant human KRT14 fragment (around aa350-472) (exact sequence is proprietary)

Clone: MSVA-614R

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Tonsil: A strong KRT14 immunostaining should be seen in the surface and crypt epithelium.

Negative Control: Tonsil: KRT14 immunostaining should be absent in all non-epithelial cells.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for KRT14

Target: KRT14 (Keratin 14 (KRT14))

Alternative Name: Cytokeratin 14 (KRT14 Products)

Synonyms: CK14 antibody, EBS3 antibody, EBS4 antibody, K14 antibody, NFJ antibody, AI626930 antibody, Krt-1.14 antibody, Krt1-14 antibody, Ka14 antibody, ouro2 antibody, keratin 14 antibody, keratin 14, type I L homeolog antibody, KRT14 antibody, Krt14 antibody, krt14.L antibody

Background: CK-14, Dowling Meara, ebs3, ebs4, Epidermolysis Bullosa Simplex, Keratin Type I Cytoskeletal 14, Koebner, NFJ,Cytokeratin 14 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P02533