Recombinant MUC1 antibody

Catalog No. ABIN7477525

This anti-MUC1 antibody is a Rabbit Monoclonal antibody detecting MUC1 in IHC. Suitable for Human.

Quick Overview for Recombinant MUC1 antibody (ABIN7477525)

Target: MUC1 (Mucin 1 (MUC1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This MUC1 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-672R

Product Details anti-MUC1 Antibody

Immunogen: Recombinant human MUC1 protein

Isotype: IgG

Application Details

Application Notes: IHC 1:75-1:150

Comment:

Positive Control: Kidney: Distal tubuli and collecting ducts must show a strong cytoplasmic MUC1 staining while the staining should be at least moderate in the parietal and visceral sheets of the Bowman's capsule and largely absent in proximal tubuli.

Negative Control: Colon: MUC1 staining should be very weak or absent in colonic epithelium.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for MUC1

Target: MUC1 (Mucin 1 (MUC1))

Alternative Name: MUC1 / EMA

Background: Breast carcinoma-associated antigen DF3, CA15-3, Carcinoma- associated mucin Episialin, Epithelial Membrane Antigen (EMA), H23AG, KL-6, MAM6, MUC-1, MUC-1/SEC, MUC-1/X, MUC1- alpha, MUC1-beta, MUC1-CT, MUC1-NT, MUC1/ZD, Mucin 1 cell surface associated, Mucin-1 subunit beta, Peanut-reactive urinary mucin, PEM, PEMT, Polymorphic epithelial mucin, PUM, Tumor-associated epithelial membrane antigen,MUC1 / EMA antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P15941

Pathways: Negative Regulation of intrinsic apoptotic Signaling