Recombinant p53 antibody

Catalog No. ABIN7477547

The Rabbit Monoclonal anti-p53 antibody has been validated for IHC. It is suitable to detect p53 in samples from Human.

Quick Overview for Recombinant p53 antibody (ABIN7477547)

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This p53 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-053R

Product Details anti-p53 Antibody

Immunogen: Recombinant human full-length TP53 protein

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Tonsil: More than 20 % of germinal centre B-cells must show a weak to moderate nuclear staining.

Negative Control: Colon: Luminal epithelial cells must remain p53 negative.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for p53

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Alternative Name: p53

Background: Antigen NY-CO-13, BCC7, Cellular Tumor Antigen p53, LFS1, TP53, Transformation Related Protein 53 (TRP53), Tumor Protein p53, Tumor Suppressor p53,p53 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P04637

Pathways: p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect