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Recombinant p53 antibody (full length)

The Rabbit Monoclonal anti-p53 antibody has been validated for IHC. It is suitable to detect p53 in samples from Human.
Catalog No. ABIN7470654
$544.93
Plus shipping costs $50.00
Shipping to: United States
Delivery in 6 to 8 Business Days

Quick Overview for Recombinant p53 antibody (full length) (ABIN7470654)

Target

See all p53 (TP53) Antibodies
p53 (TP53) (Tumor Protein P53 (TP53))

Antibody Type

Recombinant Antibody

Reactivity

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Human

Host

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Rabbit

Clonality

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Monoclonal

Conjugate

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This p53 antibody is un-conjugated

Application

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Immunohistochemistry (IHC)

Clone

053R
  • Binding Specificity

    • 48
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    full length

    Purpose

    p53 antibody (053R)

    Immunogen

    Recombinant human full-length TP53 protein

    Isotype

    IgG
  • Application Notes

    IHC 1:100-1:200

    Comment

    Positive Control: Tonsil: More than 20 % of germinal centre B-cells must show a weak to moderate nuclear staining.

    Product Description: p53 antibody validated for immunohistochemistry on 76 different Normal Tissues

    Negative Control: Colon: Luminal epithelial cells must remain p53 negative.

    Protocol

    Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Storage

    4 °C,-20 °C,-80 °C

    Storage Comment

    Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 ° C. Antibody is stable for 24 months. Non- hazardous.

    Expiry Date

    24 months
  • Target

    p53 (TP53) (Tumor Protein P53 (TP53))

    Alternative Name

    p53

    Background

    Gene Alias: Antigen NY-CO-13, BCC7, Cellular Tumor Antigen p53, LFS1, TP53, Transformation Related Protein 53 (TRP53), Tumor Protein p53, Tumor Suppressor p53

    UniProt

    P04637

    Pathways

    p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect
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