PAX6 antibody

Catalog No. ABIN7477553

The Mouse Monoclonal anti-PAX6 antibody has been validated for IHC. It is suitable to detect PAX6 in samples from Human.

Quick Overview for PAX6 antibody (ABIN7477553)

Target: PAX6 (Paired Box 6 (PAX6))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PAX6 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-706M

Product Details anti-PAX6 Antibody

Immunogen: Recombinant human PAX6 fragment

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Pancreas: A strong PAX6 positivity should be seen in the cells of islets of Langerhans.

Negative Control: Tonsil: PAX6 immunostaining should be absent in all cells.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for PAX6

Target: PAX6 (Paired Box 6 (PAX6))

Alternative Name: PAX6

Background: AN2, Aniridia type II protein, Oculorhombin, Paired box gene 6 (aniridia keratitis), Paired box homeotic gene 6, Paired box protein Pax-6, Sey, WAGR,PAX6 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P26367

Pathways: Peptide Hormone Metabolism, Carbohydrate Homeostasis