Recombinant Progesterone Receptor antibody (AA 1-200)

Catalog No. ABIN7477567

This anti-Progesterone Receptor antibody is a Rabbit Monoclonal antibody detecting Progesterone Receptor in IHC. Suitable for Human.

Quick Overview for Recombinant Progesterone Receptor antibody (AA 1-200) (ABIN7477567)

Target: Progesterone Receptor (PGR)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Progesterone Receptor antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-570R

Product Details anti-Progesterone Receptor Antibody

Binding Specificity: AA 1-200

Immunogen: Recombinant fragment (around aa 1-200) of human PGR (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes: IHC 1:50-1:100

Comment:

Positive Control: Uterine cervix: almost all columnar epithelial cells, basal squamous epithelial cells and most of the stromal cells must show a strong nuclear staining with only minimal cytoplasmic staining.

Negative Control: Tonsil: PR immunostaining should be absent in all epithelial and non-epithelial cells.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:50 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for Progesterone Receptor

Target: Progesterone Receptor (PGR)

Alternative Name: Progesterone Receptor

Background: R3C3, Nuclear receptor subfamily 3 group C member 3, PGR, PR, PRA, PRB, Progesterone receptor form A, Progesterone receptor form B,Progesterone Receptor antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P06401

Pathways: Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Smooth Muscle Cell Migration