UPK1A antibody (AA 114-173)

Catalog No. ABIN7477611

This Mouse Monoclonal antibody specifically detects UPK1A in IHC. It exhibits reactivity toward Human.

Quick Overview for UPK1A antibody (AA 114-173) (ABIN7477611)

Target: UPK1A (Uroplakin 1a (UPK1A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This UPK1A antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-735M

Product Details anti-UPK1A Antibody

Binding Specificity: AA 114-173

Immunogen: Recombinant fragment (around aa 114-173) of human Uroplakin 1A (UPK1A) protein (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Urinary bladder: A strong membranous and cytoplasmic Upk1a immunostaining should be seen in the urothelium (the staining can be limited to the top 1/3 or 2/3 of the urothelium).

Negative Control: Colon: Upk1a immunostaining should be absent in all cells of the colon mucosa.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for UPK1A

Target: UPK1A (Uroplakin 1a (UPK1A))

Alternative Name: Uroplakin 1A

Background: UP1A, UPIA, UPKA, TSPAN21,Uroplakin 1A antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: O00322