Uroplakin 1B antibody (AA 109-229)

Catalog No. ABIN7477613

The Mouse Monoclonal anti-Uroplakin 1B antibody has been validated for IHC. It is suitable to detect Uroplakin 1B in samples from Human.

Quick Overview for Uroplakin 1B antibody (AA 109-229) (ABIN7477613)

Target: Uroplakin 1B (UPK1B)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Uroplakin 1B antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-734M

Product Details anti-Uroplakin 1B Antibody

Binding Specificity: AA 109-229

Immunogen: Recombinant fragment (around aa 109-229) of human Uroplakin 1B (UPK1B) protein (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Urinary bladder: A strong membranous and cytoplasmic UPK1b immunostaining should be seen in the urothelium (the staining can be limited to the top cell layers or equally involve all cell layers).

Negative Control: Colon: UPK1b immunostaining should be absent in all cells of the colon mucosa.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for Uroplakin 1B

Target: Uroplakin 1B (UPK1B)

Alternative Name: Uroplakin 1B

Background: Tetraspanin-20, Tspan-20, TSPAN20, UP1b, UPIb, UPK1B, Uroplakin-1b,Uroplakin 1B antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: O75841