mCherry antibody

Catalog No. ABIN7862535

The Mouse Monoclonal anti-mCherry antibody is suitable to detect mCherry in samples from Various Species. It has been validated for WB.

Quick Overview for mCherry antibody (ABIN7862535)

Target: mCherry (mCherry Fluorescent Protein)

Reactivity: Various Species

Host: Mouse

Clonality: Monoclonal

Conjugate: This mCherry antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-mCherry Antibody

Purpose: Mouse Anti-mCherry Monoclonal Antibody

Specificity: The antibody detects mCherry and mCherry tag fusion proteins.

Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Immunogen: Recombinant Protein

Isotype: IgG1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:5000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.5 % BSA, 0.02 % sodium azide as Preservative and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for mCherry

Target: mCherry (mCherry Fluorescent Protein)

Alternative Name: mCherry

Background: MCherry, mCherry tagmCherry is derived from proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals), and is used as a fluorescent tracer in trasfection and transgenic experiments. The prototype for these fluorescent proteins is Green Fluorescent Protein (GFP), which is a ~27 kDa protein isolated originally from the jellyfish Aequoria victoria. The mCherry protein is derived from DsRed, a red fluorescent protein related to GFP isolated from so-called disc corals of the genus Discosoma.