BCL6 antibody (N-Term)

Catalog No. ABIN7538809

This Mouse Monoclonal antibody specifically detects BCL6 in IHC and IHC (p). It exhibits reactivity toward Human.

Quick Overview for BCL6 antibody (N-Term) (ABIN7538809)

Target: BCL6 (B-Cell CLL/lymphoma 6 (BCL6))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BCL6 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: LN22

Product Details anti-BCL6 Antibody

Binding Specificity: N-Term

Purpose: Liquid Mouse Monoclonal Antibody Bcl-6

Specificity: Human Bcl-6 Oncoprotein.

Purification: Tissue culture supernatant

Immunogen: Prokaryotic recombinant protein corresponding to 1-350 amino acids of the N-terminus of the Bcl-6 human oncoprotein molecule.

Isotype: IgG2b

Application Details

Application Notes: Immunohistochemistry on paraffin sections. Heat Induced Epitope Retrieval (HIER): Please follow the instructions for use in Novocastra Epitope Retrieval Solution pH 9. Technical note: The use of H2O2 to block endogenous peroxidase has a detrimental effect on the epitope recognized. Solutions containing 1 % or greater H2O2 can dramatically reduce the level of staining achievable. Blocking after unmasking can also lead to negative results. Therefore, it is recommended that endogenous peroxidase should be blocked before unmasking with 0.5 % H2O2 /methanol for 10 minutes. If this recommendation is not followed, staining intensity may be reduced. Suggested dilution : 1:60 for 30 minutes at 25 °C. This is provided as a guide and users should determine their own optimal working dilutions. Western Blotting: Not recommended.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid tissue culture supernatant containing sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8 °C. Do not freeze. Return to 2-8 °C immediately after use. Do not use after expiration date indicated on the vial label. Storage conditions other than those specified above must be verified by the user.

Expiry Date: 12 months

Target Details for BCL6

Target: BCL6 (B-Cell CLL/lymphoma 6 (BCL6))

Alternative Name: Bcl-6

Pathways: Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus