Cyclin D1 antibody

Catalog No. ABIN7538873

The Mouse Monoclonal anti-Cyclin D1 antibody has been validated for IHC, IHC (p) and HIER. It is suitable to detect Cyclin D1 in samples from Human.

Quick Overview for Cyclin D1 antibody (ABIN7538873)

Target: Cyclin D1 (CCND1)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cyclin D1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Heat-induced Epitope Retrieval (HIER)

Clone: P2D11F11

Product Details anti-Cyclin D1 Antibody

Purpose: Liquid Mouse Monoclonal Antibody Cyclin D1

Specificity: Human cyclin D1 protein.

Characteristics: This product is intended for the qualitative identification by light microscopy of Cyclin D1 Molecules in paraffin sections.

Purification: Tissue culture supernatant

Immunogen: Prokaryotic fusion protein corresponding to the human cyclin D1 molecule 5 .

Isotype: IgG2a

Application Details

Application Notes: Immunohistochemistry on paraffin sections. Heat Induced Epitope Retrieval (HIER): Epitope Retrieval Solution pH 9. Suggested dilution: 1:50 for 30 minutes at 25 °C. This is provided as a guide and users should determine their own optimal working dilutions. Immunohistochemical (IHC) staining techniques allow for the visualization of antigens via the sequential application of a specific antibody to the antigen (primary antibody), a secondary antibody to the primary antibody and an enzyme complex with a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. The specimen may then be counterstained and coverslipped. Results are interpreted using a light microscope.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 19 mg/L

Buffer: This product is a liquid tissue culture supernatant containing sodium azide as a preservative.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8 °C. Do not freeze. Return to 2-8 °C immediately after use. Do not use after expiration date indicated on the vial label. Storage conditions other than those specified above must be verified by the user.

Expiry Date: 12 months

Target Details for Cyclin D1

Target: Cyclin D1 (CCND1)

Alternative Name: Cyclin D1

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, ER-Nucleus Signaling