EZH2 antibody (N-Term)

Catalog No. ABIN7538887

This Mouse Monoclonal antibody specifically detects EZH2 in IHC, IHC (p) and HIER. It exhibits reactivity toward Human.

Quick Overview for EZH2 antibody (N-Term) (ABIN7538887)

Target: EZH2 (Enhancer of Zeste Homolog 2 (EZH2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EZH2 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Heat-induced Epitope Retrieval (HIER)

Clone: 6A10

Product Details anti-EZH2 Antibody

Binding Specificity: N-Term

Purpose: Liquid Mouse Monoclonal Antibody EZH2 (Enhancer of Zeste Homolog 2 (Drosophila))

Specificity: Human EZH2 (Enhancer of Zeste Homolog 2 (Drosophila)), (85 kD). Region common to isoforms A and B of the protein.

Characteristics: This product is intended for the qualitative identification by light microscopy of EZH2 (Enhancer of Zeste Homolog 2 (Drosophila)) molecules in paraffin sections.

Purification: Tissue culture supernatant

Immunogen: Prokaryotic recombinant protein corresponding to a portion of the N-terminus domain of the human EZH2 molecule.

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on paraffin sections. Suggested dilution: 1:200 for 30 minutes at 25°C. Heat induced epitope retrieval using Epitope Retrieval Solution pH 6.0. This is provided as a guide and users should determine their own optimal working dilutions.,Immunohistochemical (IHC) staining techniques allow for the visualization of antigens via the sequential application of a specific antibody to the antigen (primary antibody), a secondary antibody to the primary antibody and an enzyme complex with a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. The specimen may then be counterstained and coverslipped. Results are interpreted using a light microscope.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 20 mg/L

Buffer: This product is a liquid tissue culture supernatant containing 15 mM sodium azide as a preservative.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8 °C. Do not freeze. Return to 2-8 °C immediately after use. Do not use after expiration date indicated on the vial label. Storage conditions other than those specified above must be verified by the user.

Expiry Date: 12 months

Target Details for EZH2

Target: EZH2 (Enhancer of Zeste Homolog 2 (EZH2))

Alternative Name: EZH2)

Pathways: Retinoic Acid Receptor Signaling Pathway, Regulation of Muscle Cell Differentiation