MSH2 antibody

Catalog No. ABIN7538912

The Mouse Monoclonal anti-MSH2 antibody has been validated for IHC, IHC (p) and HIER. It is suitable to detect MSH2 in samples from Human.

Quick Overview for MSH2 antibody (ABIN7538912)

Target: MSH2 (Mismatch Repair Protein 2 (MSH2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MSH2 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Heat-induced Epitope Retrieval (HIER)

Clone: ES05

Product Details anti-MSH2 Antibody

Purpose: Liquid Mouse Monoclonal Antibody Mismatch Repair Protein (MLH1)

Specificity: Human Mismatch Repair Protein (MLH1) molecule.

Characteristics: This product is intended for the qualitative identification by light microscopy of Mismatch Repair Protein (MLH1) molecules in paraffin sections.

Purification: Tissue culture supernatant

Immunogen: Prokaryotic recombinant protein corresponding to 210 amino acids of human MLH1.

Isotype: IgG1

Application Details

Application Notes: Heat Induced Epitope Retrieval (HIER): Epitope Retrieval Solution pH 6. Suggested dilution: 1:50 for 30 minutes at 25 °C. This is provided as a guide and users should determine their own optimal working dilutions. The performance of this antibody should be validated when utilized with other manual staining systems or automated platforms. Immunohistochemical (IHC) staining techniques allow for the visualization of antigens via the sequential application of a specific antibody to the antigen (primary antibody), a secondary antibody to the primary antibody and an enzyme complex with a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. The specimen may then be counterstained and coverslipped. Results are interpreted using a light microscope.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 165 mg/L

Buffer: This product is a liquid tissue culture supernatant containing sodium azide as a preservative.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8 °C. Do not freeze. Return to 2-8 °C immediately after use. Do not use after expiration date indicated on the vial label. Storage conditions other than those specified above must be verified by the user.

Expiry Date: 12 months

Target Details for MSH2

Target: MSH2 (Mismatch Repair Protein 2 (MSH2))

Alternative Name: Mismatch Repair Protein

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response